the tricky part is separating the strands, then isolating the strand
A much much much easier method is to make riboprobes.
Just PCR your 200 bp piece of DNA with primers that contain T7 and T3
promoters as overhangs.
By in vitro transcription with T7 or T3 RNA polymerase, you can make
sense or antisense probes. Simply label them by adding
radioactive/fluorescent/DIGged/biotinylated/etc. rNTPs to your
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