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E. coli lysis for Protein Isolation- URGENT - CORRECTION

mb mb at laplace.csb.yale.edu
Thu Jan 20 15:24:56 EST 2000


possiblities for cell lysis include:

non-ionic detergents like thesit @1%
lysozyme @ 0.1 -1 mg/mL
multiple cycles of freeze thaw in dry ice/EtOH or liquid nitrogen

try following one of these with multiple passes through a narrow bore
syringe.

--
Mark Bowen
Research Associate
HHMI/Yale University
Dept. of Molecular Biophysics and Biochemistry


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