production of antisense oligonucleotide by PCR ?

R. John Lye rjl6n at
Thu Jan 20 15:10:41 EST 2000

rogier wrote: 
> the tricky part is separating the strands, then isolating the strand
> you need.

Not really - not if you do a linear amplification using a
single primer.  I don't have any idea how well it would
work as an antisense oligo, but you'd get only one strand.
John Lye
rjl6n at

More information about the Methods mailing list