unspecific RT-PCR line

Bethune International Peace Hospital genet at public.sj.he.cn
Thu Jan 20 20:08:28 EST 2000


Dear netter
I use  single tube RT-PCR protocol to detect GAPDH mRNA .
the PCR products is a weak 309bp aim line and a strong line 
of 100bp(unexpected) or so . why? How to improve my system?.
Any suggestions would be gratefully received!
Yours,
jiao
reply to :
genet at public.sj.he.cn

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