Transformation problems...

R. Jayakumar jakku at mrna.tn.nic.in
Fri Jan 21 10:23:08 EST 2000


----- Original Message -----
From: Jan-Henner Wurmbach <wurmbach at uke.uni-hamburg.de>
To: <methods at hgmp.mrc.ac.uk>
Sent: Monday, January 17, 2000 9:10 PM
Subject: Re: Transformation problems...


> "R. Jayakumar" <jakku at mrna.tn.nic.in> schrieb im Beitrag
> <004601bf6031$e485c820$8c01a8c0 at tn.nic.in>...
> [snip]
>
> >     ONE STEP TRANSFORMATION PROTOCOL FOR E.COLI
> [snip ]
> > 5. Take a 100ul aliquot and add DNA (around 10-20ng) and leave on ice
for
> > 30min (we found that heat shock treatment gives less transformants than
> ice
> > treatment).
> Interesting! In our lab Heat shock is needed, at least with ligations. For
> supercoiled Plasmids there seems to be no difference. Maybe plenty of DNA
> and HS is giving an efficiency that is inhibiting?
>
> Greetings
>   Jan-Henner Wurmbach <wurmbach at uke.uni-hamburg.de>
>
> [snip rest of mail]
>

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