----- Original Message -----
From: Jan-Henner Wurmbach <wurmbach at uke.uni-hamburg.de>
To: <methods at hgmp.mrc.ac.uk>
Sent: Monday, January 17, 2000 9:10 PM
Subject: Re: Transformation problems...
> "R. Jayakumar" <jakku at mrna.tn.nic.in> schrieb im Beitrag
> <004601bf6031$e485c820$8c01a8c0 at tn.nic.in>...
>> > ONE STEP TRANSFORMATION PROTOCOL FOR E.COLI
> [snip ]
> > 5. Take a 100ul aliquot and add DNA (around 10-20ng) and leave on ice
> > 30min (we found that heat shock treatment gives less transformants than
> > treatment).
> Interesting! In our lab Heat shock is needed, at least with ligations. For
> supercoiled Plasmids there seems to be no difference. Maybe plenty of DNA
> and HS is giving an efficiency that is inhibiting?
> Jan-Henner Wurmbach <wurmbach at uke.uni-hamburg.de>
>> [snip rest of mail]