> In article <200001201835.TAA01546 at intwww.zit.med.uni-tuebingen.de>,
>Wolfgang.Schechinger at med.uni-tuebingen.de ("Wolfgang Schechinger") wrote:
>> > Wher might I obtain a cDNA of active Factor Xa from?
> > I couldn't find a clone at ATCC (i expected to do).
>> why not look for the cDNA for Factor X. If you look at the coagulation
> cascade, you will see that FX is activated to FXa by the prothrombinase
> complex which can be Factor Va: factor VIIa, Tissue factor: VIIa, and EPR:
> and as yet unidentified protein.
>> In other words, Factor X is proteolitically cleaved to form factor Xa.
>> Just out of curiosity, why are you interested? Factor X is made only by
I imagine he wants to make it in bacteria to use for cleaving GST tags off of
fusion proteins. It very expensive to buy, aty least it used to be..