Factor Xa cDNA

Colin Rasmussen colin at pombe.usask.ca
Fri Jan 21 13:58:57 EST 2000



pathos wrote:

> In article <200001201835.TAA01546 at intwww.zit.med.uni-tuebingen.de>,
> Wolfgang.Schechinger at med.uni-tuebingen.de ("Wolfgang Schechinger") wrote:
>
> > Wher might I obtain a cDNA of active Factor Xa from?
> > I couldn't find a clone at ATCC (i expected to do).
>
> why not look for the cDNA for Factor X.  If you look at the coagulation
> cascade, you will see that FX is activated to FXa by the prothrombinase
> complex which can be Factor Va: factor VIIa, Tissue factor: VIIa, and EPR:
> and as yet unidentified protein.
>
> In other words, Factor X is proteolitically cleaved to form factor Xa.
>
> Just out of curiosity, why are you interested?  Factor X is made only by
> hepatocytes.

I imagine he wants to make it in bacteria to use for cleaving GST tags off of
fusion proteins.  It very expensive to buy, aty least it used to be..

Colin Rasmussen





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