IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

DNA/protein mobility shift assay

Mark Schoenbeck mark at capsidiol.ca.uky.edu
Fri Jan 21 16:56:25 EST 2000

I am preparing to look at protein binding to regions of promoter DNA.  I
would appreciate hearing from anyone who can suggest a systematic method
for optimizing protein binding conditions for a gel mobility shift
assay.  A previous lab member here used nuclear and whole-cell extracts
and allowed 10 minutes for binding before running the labeled DNA
through a 12% acrylamide 0.5 x TBE buffered gel.  Can anyone tell me if
this is even a suitable buffer for retaining protein-DNA interactions?

Thanks in advance for any assistance you can offer,

Mark Schoenbeck
University of Kentucky

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net