phenol and cresol assay

Champreda, Verawat verawat.champreda at ic.ac.uk
Sat Jan 22 11:30:44 EST 2000


Dear everybody,
        I want to assay oxidase activity of an oxygenase enzyme from
bacteria. This enzyme oxidizes benzene to phenol and toluene to a mixture of
cresols (o-, m- ,p-). The assay method we used now is based on GC assay but
this is laborious. I would like to know whether there are any sensitive
colourimetric or fluorometric assay for detection of phenol and cresol.
        Thank you for your attention.

Yours sincerely,

Verawat Champreda
Department of Biochemistry
Imperial College of Science, Technology and Medicine
LondonSW7 2AY, UK 
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