SURE2 cells' transformation efficiency?
polliwog at u.washington.edu
Sat Jan 22 22:22:45 EST 2000
Does anyone out there have any experience with Stratagene's
(chemical-)competent SURE2 cells?
Following the (routine) protocol I have gotten poor transformation
efficiency (like 10e2/ug) from two different batches. Do these cells go
bad more quickly than other standard strains?
Alternatively, can someone recommend a strain which can tolerate
large hairpin constructs without deletion?
Seth D. Findley polliwog at u.washington.edu
Department of Biochemistry Phone: 206-543-1710
Health Sciences Building J579
University of Washington, Box 357350, Seattle, WA 98195
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