Hi
I have a problem. I got a 1kb insert cloned into a pGEM-T vector. The
insert contains two BamHI sites which need to be removed. Can anyone
suggest a suitable strategy for this. The insert does not encode a protein
and is actually an intron.
I have already tried 1) Klenow endfilling of BamHI cohesive ends 2) Iam
planning to remove these sites by PCR invitro-mutagenesis. Since this is a
bit costly, It would be nice if I could get some economical means of doing
it. Any adapters for this?
thanking everyone in advance.
jayakumar
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||R. JAYAKUMAR
CSIR- Senior Research Fellow
Dept. of Molecular Microbiology,
School of Biotechnology,
Madurai Kamaraj University,
Madurai - 625 021.
India
email: jakku at usaf.org
tel: +91-452-858471-374
efax: (603)-688-4665
web: http://members.tripod.com/~jakspage
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