How do you eliminate a RE site

[Dr. Duncan Clark]

In article <000901bf6572$4c13b3e0$8c01a8c0 at tn.nic.in>, R. Jayakumar
<jakku at mrna.tn.nic.in> writes
>I have already tried 1) Klenow endfilling of BamHI cohesive ends 2) Iam
>planning to remove these sites by PCR invitro-mutagenesis. 


Or T4 DNA pol to remove the overhangs rather than gap fill them in.

Duncan

PS Suggestion:


Sticking a hyphen hyphen space before your very long signature file will
have it automatically removed by most newsreader software rather than
beong re-quoted i.e 

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>R. JAYAKUMAR
>CSIR- Senior Research Fellow
>Dept. of Molecular Microbiology,
>School of Biotechnology,
>Madurai Kamaraj University,
>Madurai - 625 021.
>India
>email: jakku at usaf.org
>tel: +91-452-858471-374
>efax: (603)-688-4665
>web: http://members.tripod.com/~jakspage
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>"There is a possible even in imPOSSIBLE"
>
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-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk