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antibody purification

Marianne Schwartz bio-a079 at post6.tele.dk
Mon Jan 24 07:27:35 EST 2000

I have raised antibody against a protein, which was purified on a Ni2+
column, after expressed in E.coli. (has His TAGs in the N-terminal). The
protein looked perfectly pure on a gel, however, the antibody recognize
some E.coli proteins also, as judge from a Western. . Can I get rid of
these antibodies ?
Does anybody has a protocol for this problem.


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