antibody purification

Lee Hunt L.hunt at sheffield.ac.uk
Mon Jan 24 08:03:35 EST 2000



You can make an affinity column using proteins isolated from your bacterial
strain without your his-tagged protein. Run your antisera down this and all
these contaminants should be removed. A cruder method is to make an acetone
powder from bacteria without your protein and just mix it with your
antisera.
There are methods for this in Maniatis and in Harlow & Lanes book
"Antibodies"





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