Does anyone know how PCR errors in a targeting vector would effect
I have an SV129 mouse Genomic phage clone, that I have been unable to
I have experience "PCRing" the targeting region and would like to PCR
and subclone this for the targeting vector.
With pfu or some of the other higher fidelity Taqs, I could achieve an
error rate of less than 1/500 bps.
There might be 20 nucleotide differences in a 10 kb insert.
Any references or experience with using PCR to generate KO targeting
vectors would be greatly appreciated.
Thank you in advance for your time,
Ph.D. Candidate in MCBB
353-5311, vfunari at bio.bu.edu