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Primer extension

Sergio sergioal at solea.quim.ucm.es
Thu Jan 20 12:37:40 EST 2000

lumdicks wrote:

> Hi,
>       I've encountered some problems in performing primer extension. It
> is that I only get smear in the X-ray film instead of the band. So, what
> is the problem of this?? Besides, is the salt in the extension reaction
> affect gel electrophoresis?? Because my sequencing gel run very strange
> and I suspect the problem is due to the excessive salt in the reaction.
> Thanks in advance.
> Regards,
> Lum

Hi Lum. As you suspect, the salt concentration affects the electrophoresis. I
don't know the protocol you are using, but if you are interested i could send
you the mine, which is working ok.

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