>> I've encountered some problems in performing primer extension. It
> is that I only get smear in the X-ray film instead of the band. So, what
> is the problem of this?? Besides, is the salt in the extension reaction
> affect gel electrophoresis?? Because my sequencing gel run very strange
> and I suspect the problem is due to the excessive salt in the reaction.
>> Thanks in advance.
Hi Lum. As you suspect, the salt concentration affects the electrophoresis. I
don't know the protocol you are using, but if you are interested i could send
you the mine, which is working ok.