production of antisense oligonucleotide by PCR ?

Warren watchcity at my-deja.com
Mon Jan 24 16:24:58 EST 2000


Hello, Are you familiar with the HighPure BMB gel iso kit?  You can do a PCR
from your template, run out on a TAE (2+%) gel, and cut out the 200 bp band. 
The HighPure is preferable to (This was years ago) non-size selective silicon
matrix DNA purification kits (Promega?).  Do a double chaotropic soln. wash
if concerned about purity.  Elute in buffer ofyour choice.  Cheers,  Warren


In article <3.0.1.32.20000119174016.006a66a0 at cmu.unige.ch>, 
Jacqueline.Fischer-Lougheed at medecine.unige.ch (Jacqueline Fischer-Lougheed)
wrote:

> Has anyone ever tried to produce large amounts of single-stranded
> oligonucleotides (about 200 bases long) for ANTISENSE technology using PCR
> on a linearized vector?
> I thank you in advance for references or protocols (or reasons why this
> idea is no good!).
>
> **********************************************************************
> Jacqueline Fischer-Lougheed, PhD
> Physiology Department / C.M.U. (University of Geneva Medical School)
> 1 rue Michel-Servet
> 1211 GENEVA 4
> Switzerland
>
> tel. (+41) (22) 702.53.95 - fax. (+41) (22) 702.54.02
> e-mail:  Jacqueline.Fischer-Lougheed at medecine.unige.ch
> **********************************************************************
>
> ---
>


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