Hello, I am a graduate student at Colorado State University and I am
looking for a protocol for driving specific, single round trascription
by RNA Polymerase I by using heparin. Our lab has traditionally used
sarkosyl but for what I wish to do sarkosyl will not work. One of the
professors here recommended heparin but I am having a hard time finding
a protocol for its use in single round transcription for any RNA
polymerase. Any help would be appreciated.
sfields at lamar.colostate.edu