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SYBR Gold Cloning

Chris Small chris at genomex.com
Tue Jan 25 15:43:34 EST 2000


Hi,

I was wondering whether anyone has experience staining high MW DNA 
with SYBR Gold, visualizing it with the "Dark Reader" then isolating 
it from the gel and cloning it.  Some literature says it doesn't 
damage the DNA (like ETBR) and subsequently affect cloning efficiency 
but I would like hear it from someone that has tried it.

Thanks in advance!

C

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