lumdicks wrote:
> Hi,
>> I'm planning to use slot blot hybridization to calculate the gene
> copy number in the genome. However, my slot blot give a very weak signal
> even 50ug of genomic DNA was applied. Is there any possible casue for
> this..?? I am using DIG labelling and chemiluminecence detection. The
> system works fine for southern blot (using the same probe and genomic
> DNA), which can detect a single copy gene using 5 - 10ug of genomic DNA.
> So, I don't know what's wrong with the slot blot. Can anyone give any
> suggestion??
Are you denaturing your genomic DNA before you apply it to the filter?
Colin Rasmussen
