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Gene copy number by slot-blot hybridization

Colin Rasmussen colin at pombe.usask.ca
Wed Jan 26 11:43:30 EST 2000



lumdicks wrote:

> Hi,
>
>       I'm planning to use slot blot hybridization to calculate the gene
> copy number in the genome. However, my slot blot give a very weak signal
> even 50ug of genomic DNA was applied. Is there any possible casue for
> this..?? I am using DIG labelling and chemiluminecence detection. The
> system works fine for southern blot (using the same probe and genomic
> DNA), which can detect a single copy gene using 5 - 10ug of genomic DNA.
> So, I don't know what's wrong with the slot blot. Can anyone give any
> suggestion??

Are you denaturing your genomic DNA before you apply it to the filter?

Colin Rasmussen





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