drying glycine gels
Chris H Lindley
chris at scotgate2.demon.co.uk
Tue Jan 25 17:35:34 EST 2000
On Sat, 22 Jan 2000 07:52:00 -0600, jdeleha at mailhost.tcs.tulane.edu wrote:
>I had a similar problem with gels cracking on our BioRad gel dryer and
>adding glycerol after destaining and fixing couldn't alleviate the problem
>completely as some gels still cracked (probably because our house vacuum is
>not strong enough).
>I came across a kit from Novex (division of Invitrogen) called the Gel-Ease
>gel drying system which dries the gel between two pieces of cellophane
>overnight at room temp in an EtOH-based drying solution. The end product is
>a nice laminated gel that is absolutely beautiful. I just used it for the
>first time and the results are incredible.
I've used a similar system, and it's nice. But remember 35S's signal will be
ever so weak after coming through this!
(If you're doing autorads that is!)
>I hate to endorse kits but gels are a big time investiment and it breaks my
>heart after all that work only to have them crack. I would recommend this
>method of drying to anyone.
I use thin gels(0.8mm I think) and they hardly ever crack. This is
straight after running them, without staining.
>A.F. Simpson wrote in message <3888A8F6.70FD at le.ac.uk>...
>>> I am trying to find info about drying small glycine gels (running small
>>> peptides on the gels) in standard gel dryers because I have been having
>>> continuing problems with my gels cracking. Does anyone know where I
>>> find a comprehensive reviews or protocols for gel drying --other than the
>>> dryer's manual?
>>I don't know about a manual, but have your tried soaking overnight in
>>destain with 3% glycerol after fixing? That solved my problems with
¦ Chris H. Lindley Yorkshire, UK ¦
¦ chris at scotgate2.demon.co.uk Ferg on #os/2 and #os2uk, EFnet ¦
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¦ Molecular Biology & OS/2 www.scotgate.demon.co.uk ¦
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