I have a problem with my GST-pulldown assay for interacting
domain mapping. My problem is that in vitro translated input
proteins(smaller than 25kDA) always gives me fuzzy bands.
But I can get clear signals after binding to beads.
I used 15% SDS PAGE.
Any ideas would be appreciated
Thanks in advance.
The reasonable man adapts himself to the world; the unreasonable
one persists in trying to adapt the world to himself. Therefore,
all progress depends on the unreasonable man.