(Q) in vitro translation of small protein
gidarim at family.sogang.ac.kr
Thu Jan 27 01:50:00 EST 2000
I have a problem with my GST-pulldown assay for interacting
domain mapping. My problem is that in vitro translated input
proteins(smaller than 25kDA) always gives me fuzzy bands.
But I can get clear signals after binding to beads.
I used 15% SDS PAGE.
Any ideas would be appreciated
Thanks in advance.
The reasonable man adapts himself to the world; the unreasonable
one persists in trying to adapt the world to himself. Therefore,
all progress depends on the unreasonable man.
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