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(Q) in vitro translation of small protein

Kim, Chun gidarim at family.sogang.ac.kr
Thu Jan 27 01:50:00 EST 2000


I have a problem with my GST-pulldown assay for interacting 
domain mapping. My problem is that in vitro translated input 
proteins(smaller than 25kDA) always gives me fuzzy bands. 
But I can get clear signals after binding to beads. 
I used 15% SDS PAGE.

Any ideas would be appreciated
Thanks in advance.

     The reasonable man adapts himself to the world; the unreasonable
     one persists in trying to adapt the world to himself. Therefore,
     all progress depends on the unreasonable man.

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