drying glycine gels

Richard J. Dudley rdudley at pitt.edu
Thu Jan 27 08:25:18 EST 2000


If you're running a high percentage glycine gel (>10-12%), try using FMC's Long
Ranger or Amresco's PAGE-Plus.  I had a terrible time with 15% gels cracking,
even with glycerol, until I switched to the PAGE-Plus.  Also, if you're
separating small peptides, think about a tricine buffer?

rich

"A.F. Simpson" wrote:

> Erin_Flanagan wrote:
> >
> > I am trying to find info about drying small glycine gels (running small
> > peptides on the gels) in standard gel dryers because I have been having
> > continuing problems with my gels cracking.  Does anyone know where I could
> > find a comprehensive reviews or protocols for gel drying --other than the
> > dryer's manual?
>
> I don't know about a manual, but have your tried soaking overnight in
> destain with 3% glycerol after fixing?  That solved my problems with
> cracking gels.
>
> > Thanks
> > erin
>
> love
> Anna

--
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Cystic Fibrosis Research Center
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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