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dephosphorylate a protein in cell lysate

Stephanie Toering stoering at stanford.edu
Thu Jan 27 13:35:20 EST 2000


You're going to have a tough time dephosphorylating a protein
with a *nucleic acid* phosphatase.  Try potato acid phosphatase
(available from Roche).  You probably won't get complete
dephosphorylation in complete lysate, but I think you'll see
some kind of change on the Western.




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