Gene copy number by slot-blot hybridization

John Lum johshi at hotmail.com
Thu Jan 27 23:38:33 EST 2000


Hi,

>     How do you fix the DNA on the membrane? Sometimes, just drying the membrane
> would be enough; but if you want a better fixation you may try to use UV
> irradiation or heat fixation (placing the membrane in an incubator at 80ºC for
> 1.5-2 hours would do both a partial denaturation of the DNA and fixation to the
> membrane).

      I've try both UV-fixation and baking at 120C for 40 mins. Both of them have
similar result.

Regards,
Lum





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