Nonidet P40 and Bradford

Mano mdep at musica.mcgill.ca
Fri Jan 28 23:35:40 EST 2000


Hi there,
I have had the same question in the past, but did not investigate it too
much.
NP-40 or IGEPAL works equally well for me.  When NP40 was no longer
available, I tried IGEPAL and it worked fine, but I did not compare the two
for efficiency, since they are "chemically" identical anyways.
    In terms of the Bradford assay from BIO RAD, it also works fine.  I use
1-3 microlitres and never seen a problem.
Take care,
Mano



"Dr. Fernando Rodriguez-Pascual" wrote:

> Hi all,
>
> Have any of you experienced problems in performing protein
> determinations by Bradford using extracts containing 0.5% Nonidet P40. I
> mean, whether it is compatible with the Bradford reagent and up to which
> concentration. In former times I used to have no problems for that but
> now I find that anyhow it affects the assay.
> By the way, Sigma is not longer purchasing Nonidet P40, but a
> "chemically indistinguible from it" compound (as Sigma) called IGEPAL-CA
> 630. Do you know if it is really the same? Have you found problems by
> using this new nonionic detergent?
>
> Chao
>
> Fernando
> --
> #############################################
> Dr. Fernando Rodríguez Pascual
> Department of Pharmacology
> Johannes Gutenberg University
> Obere Zahlbacher Str. 67
> D-55101 Mainz, Germany
> Tf. +49 (6131) 39 3 68 36 or 39 3 32 45
> Fax. +49 (6131) 39 3 66 11
> email: frodrigu at mail.uni-mainz.de
> #############################################





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