Yeast expression problem

Younes Bounhar ybounh at po-box.mcgill.ca
Fri Jan 28 23:56:53 EST 2000


HI all

I have cloned the gene for a lethal protein, namely bax, in yeast inducible
vector but can't get any expression. I have already done expression using
other proteins in the same vector and these work just fine. Plus I subcloned
the gene into pBKS and tried in vitro translation and it produced the right
product (as verified by immunoprecipitation)!! Does anybody have a clue?
Note that this has already been done in other labs and published so...!!!
i'm puzzled

THANX

--
Younes Bounhar
be  yourself no matter what they say
email: ybounh at po-box.mcgill.ca






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