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Large scale protein production from stably transfected cells ?

Svend Kjaer svend at cajal.mbb.ki.se
Sat Jan 29 12:01:00 EST 2000

Dear all,

I have some stably transfected HEK cells, which secrete my favorite
protein which is the extracellular part of a receptor. I want to produce
"pseudo-industrial" amounts of my protein and I was wondering how to
optimise production/secretion.
Is it best to grow the cells to a certain density and then apply
serum-free medium for a given amount of time and harvest it, or is a
continuous exchange of conditioned medium from confluent cells to prefer

Any inputs appreciated,



Svend Kjær, Ph.D.
Division of Molecular Neurobiology,
Department of Neuroscience
Karolinska Institute
Berzelius väg 1, 3rd floor
171 77 Stockholm

Tel: +46-8-7287666
Fax: +46-8-339548

Private phone: +46-8-6409962


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