pMal-c2, modification of

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Sun Jan 30 14:28:16 EST 2000


So it's probably a good idea to keep the context before the 
translation start the same as it is in original vector.

Thanks a lot!

Wolfgang

> 
> However, you should be aware that the presence of a fusion partner
> is not just only for purification.  The presence of a fusion partner
> helps with solubility, protein expression, stability, etc.  One
> thing that you should bear in mind is that without the fusion
> partner, your protein may express poorly due to inefficient
> translation initiation.  This very much depends on the initial DNA
> sequence of your gene.  A high GC content at the translation
> initiation region of your coding sequence is generally bad.
> 
> >
> > All input is welcome!
> >
> > Wolfgang

Dr. Wolfgang Schechinger, Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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