pMal-c2, modification of
Wolfgang.Schechinger at med.uni-tuebingen.de
Sun Jan 30 14:28:16 EST 2000
So it's probably a good idea to keep the context before the
translation start the same as it is in original vector.
Thanks a lot!
> However, you should be aware that the presence of a fusion partner
> is not just only for purification. The presence of a fusion partner
> helps with solubility, protein expression, stability, etc. One
> thing that you should bear in mind is that without the fusion
> partner, your protein may express poorly due to inefficient
> translation initiation. This very much depends on the initial DNA
> sequence of your gene. A high GC content at the translation
> initiation region of your coding sequence is generally bad.
> > All input is welcome!
> > Wolfgang
Dr. Wolfgang Schechinger, Pathobiochemistry Dept.
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de
*unsolicited mail is *NOT* appreciated, usual disclaimers apply
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