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Immunoprecipitation question...

M. Johan Broekman hanbroekman at csi.com
Mon Jan 31 06:58:33 EST 2000


Mailed and posted:

Neal Robert Melvin wrote:
> 
> When I've pelleted my beads during washing steps, they (obviously) all
> clump together in one big ball... is it advisable to break this clump up
> by vortexing to disperse the beads?? I'm assuming this will allow more
> efficient washings, but I'm worried that vortexing might knock my antigen
> off. Any suggestions??
> 
> Please email me directly, as  I rarely have access to newsgroups.
> 
> Thanks in advance,
> 
> Neal
> 
<snip excessive sig>

Use your forefinger and flick the tube a few times.  That should
resuspend the beads.  If your antigen would fall of the bead, you have a
very, very poor antibody.  It is much more likely the beads would fall
apart.
-- 
Best regards,
Han Broekman
(please answer to the newsgroup)




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