Mailed and posted:
Neal Robert Melvin wrote:
>> When I've pelleted my beads during washing steps, they (obviously) all
> clump together in one big ball... is it advisable to break this clump up
> by vortexing to disperse the beads?? I'm assuming this will allow more
> efficient washings, but I'm worried that vortexing might knock my antigen
> off. Any suggestions??
>> Please email me directly, as I rarely have access to newsgroups.
>> Thanks in advance,
>> Neal
><snip excessive sig>
Use your forefinger and flick the tube a few times. That should
resuspend the beads. If your antigen would fall of the bead, you have a
very, very poor antibody. It is much more likely the beads would fall
apart.
--
Best regards,
Han Broekman
(please answer to the newsgroup)
