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Kozak consensus question

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Mon Jan 31 09:27:38 EST 2000


Hi all, 
I have a squence like this: GCC GCC ACC ATG G; it contains a Kozak 
consus element in order to ensure efficient translation.

I'd like to introduce a NdeI restriction site (catatg). 

What might be the better solution:
a) mutating the sequnce to GCC GCC cat ATG G (mutations are lower 
case)

b) inserting two bases; the sequence then would be GCC GCC ACC at ATG 
G

will either of the two ways destroy the effect of the Kozak element?

Thaks for all input!

Wolfgang

Dr. Wolfgang Schechinger, Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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