Hi all,
I have a squence like this: GCC GCC ACC ATG G; it contains a Kozak
consus element in order to ensure efficient translation.
I'd like to introduce a NdeI restriction site (catatg).
What might be the better solution:
a) mutating the sequnce to GCC GCC cat ATG G (mutations are lower
case)
b) inserting two bases; the sequence then would be GCC GCC ACC at ATG
G
will either of the two ways destroy the effect of the Kozak element?
Thaks for all input!
Wolfgang
Dr. Wolfgang Schechinger, Pathobiochemistry Dept.
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
-----
*unsolicited mail is *NOT* appreciated, usual disclaimers apply
-----
Pressing ALT+F4 simultaneously enables you to read private messages
---
