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Kozak consensus question

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Mon Jan 31 09:27:38 EST 2000

Hi all, 
I have a squence like this: GCC GCC ACC ATG G; it contains a Kozak 
consus element in order to ensure efficient translation.

I'd like to introduce a NdeI restriction site (catatg). 

What might be the better solution:
a) mutating the sequnce to GCC GCC cat ATG G (mutations are lower 

b) inserting two bases; the sequence then would be GCC GCC ACC at ATG 

will either of the two ways destroy the effect of the Kozak element?

Thaks for all input!


Dr. Wolfgang Schechinger, Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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