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Lambda DNA

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Jan 31 09:33:43 EST 2000

In article <3895679E.B1E5A97B at ifremer.fr>, Jean-Paul RAFFIN
<jpraffin at ifremer.fr> writes
>Has somebody used primed-lambda DNA for DNA polemerase reactions ?
>What concentrations should be used ?

If you mean how much lambda to put in a PCR then 1ng in 50ul and
20-25cycles should be fine for up to 3kb PCR. Load 10% on an agarose gel
and you should have a good intensity band.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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