High background in Southern hybridization

Mikko Taipale mikko.taipale at helsinki.fi
Sun Jul 2 12:56:00 EST 2000


"David L Haviland" <David.L.Haviland at uth.tmc.edu> wrote:
> I've never played with dextran sulfate either.  When asked, many  years
> ago my post-doc mentor told me in no certain terms that dextran sulfate
> was a recipe for high background and not to use it.

Can you say what he meaned by high background? Is it unspecific
hybridization or just that the probe binds non-covalently all over to the 
membrane? My problem is the former, unspecific hybridization signal.

Mikko






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