metall ion affinity purification of proteins - something better
byung-hoon.kim at uni-tuebingen.de
Mon Jul 3 03:47:37 EST 2000
Wolfgang Schechinger schrieb:
> Hi all,
> are there other (competitive/better) methods for purifying proteins
> using affinity to chelated metal ions than his6 / nickel?
> all input is welcome!
as far as I know Roche puduces an alternative purification kit,
But I don't know whether it's more efficient than 6xHis/nickel system, I
havn't used it.
They say, reducing agents (DTT, beta-Mercaptoethanol) can be used during
the purifacation step, which is not the case in 6xHis/Ni system.
Univerisity of Tuebingen
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