metall ion affinity purification of proteins - something better

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Mon Jul 3 15:27:53 EST 2000


In article <200007022145.XAA09296 at intwww.zit.med.uni-tuebingen.de>, Wolfgang.Schechinger at med.uni-tuebingen.de ("Wolfgang Schechinger") wrote:
:Hi all, 
:
:are there other (competitive/better) methods for purifying proteins 
:using affinity to chelated metal ions than his6 / nickel?
:
:all input is welcome!

You can always try different metals (Co, Zn, Cu, Fe) with NTA, 
and you can always try IDA instead of NTA. There are some subtle
variations between behavior of different proteins on metal chelates, 
but generally speaking, _no_, there is no "better" method than Ni-NTA
for purification of recombinant proteins based on IMAC. 

        - Dima






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