Sv: GFP-neo fusion?

[Jens Tornoe]

>
> has anyone actually tried GFP-neo fusion? I couldnt find any info on
Medline.
> It would really be very handy, I think, as a secondary cistron for
> selection and expression-level control... (may be better than making a
> tricistronic cassette).

We have made, not a neo-EGFP, but a Zeo-EGFP fusion that works fine. I guess
it could be done with neo also without too many difficulties.

Jens

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Jens Tornøe
NsGene
Pederstrup, Denmark