metall ion affinity purification of proteins - something better
harold.taylor.remove-this at delete-this.uni-tuebingen.de
Tue Jul 4 02:57:38 EST 2000
Wolfgang.Schechinger at med.uni-tuebingen.de ("Wolfgang Schechinger")
>are there other (competitive/better) methods for purifying proteins
>using affinity to chelated metal ions than his6 / nickel?
Wolfgang, it's a question of what you're trying to do ... You can try
different metal ions or different chelates to immobilize the metal
ions or different conditions to elute your bound proteins. If you have
nothing better to do this afternoon, come across the street to the
biochemistry department (Hoppe-Seyler-Str. 4, top floor, room 415) and
we can talk about what you can try ;-) If nothing else, you can get a
cup of coffee.
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