alternatives to ethidium bromide
i.j.harvey at ncl.ac.uk
Tue Jul 4 10:26:37 EST 2000
Vasos Panagiotopoulos +1-917-287-8087 Bioengineer-Financier
<vjp2 at dorsai.org> wrote in message
news:slrn8lutmq.a4n.vjp2 at amanda.dorsai.org...
> One of my clients who felt the clock ticking on his career decided
> to skip most EtBr safety precautions. When I freaked out, he said that
> skin is mostly dead tissue and short-term contact with EtBr was
> over-exaggerated risk. That was five years ago, and despite my doubts
> his ..actions, he hasn't developed anything. Please don't follow his
> but perhaps someone might wish to discuss his comments.
I've stopped using EtBr more to avoid the sunburn than for any other reason
(I use SYBR Green and a blue transilluminator). For occasional amusement I
carry an unstained agarose gel around the department just for the reaction
from the safety officers. One of them ALWAYS takes two steps backwards and
almost screams. The blue illuminator usually gets good reactions from
people who are used to the concept that transilluminators make you go blind.
The combination does seem to help with cloning fragments isolated from gels.
Other than that I've got to agree with the poster who advocates good
practice as protection for others.
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