Universal Genome walker kit

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Tue Jul 4 18:27:26 EST 2000


Like I said, the ratio of the polymerases needs to be worked out for a
particular application. Somebody mentioned a 5:1 ratio too!

At 11:55 AM 7/4/00 +0100, Bertrand Collet wrote:
>A mix of 50 % Taq and 50 % Pfu works well in our hand, using Hot start
>(wax based or other) and Touchdown techniques.
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Dr. Hiranya Sankar Roychowdhury
Dept. of Molecular Biology	
PO Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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