S-35 and autoradiography
MICMDF at leeds.ac.uk
Wed Jul 5 07:57:45 EST 2000
In article <8jqs8v$bm0$1 at mozo.cc.purdue.edu>,
mhari at expert.cc.purdue.edu (Malathi Hari) wrote:
> I am looking for experienced opinion about exposure times for S-35
>protein labelling followed by IP and exposing to X-ray film versus
>Which of the above did u prefer and why?
>Thanks is advance.
I have used phosphorimaging for visualising 35-S labelled proteins after
separation by SDS-PAGE. I loaded approx. 20,000 cpm per lane and exposed the
gel to the imaging plate for 4 hours. I chose phosphorimaging because of its
supposed greater sensitivity and reduced exposure time, plus the image comes
up on the computer and can be easily transferred to other applications. Also,
if the signal is not strong enough the gel can be re-exposed for a longer time
period which is still quicker than using X-ray film.
Division of Microbiology
University of Leeds
micmdf at leeds.ac.uk
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