Best vector for expressing recombinant antibody fragments?

Chris LaRosa clarosa at biocomp.unl.edu
Wed Jul 5 16:00:48 EST 2000



Benny Lo wrote:
> 
> Oops, forgot one thing: I 'd like to express in E. coli preferably but don't
> mind in mammalian systems if the yields are good.
> 
> Thanks.
I dont know about best for antibodies, but the Qiagen PQE-30 worked well
for me. ok expression, and the Hisx6 tag allowed easy purification for
antibodies against the open, unfolded protein.  The documentation was
very good for the sometime protein biochemist.






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