amooser at biocfebs.unizh.ch
Thu Jul 6 04:51:39 EST 2000
I have been using the recombinant bovine enterokinase (from Invitrogen)
to cleave my fusion protein. Recognition sequence: (Asp)4-Lys
The system works almost perfectly, except that I get two proteins: mine
and mine with the first four AA deleted (Ala-Lys-Ser-Arg).
Have you ever heard of unspecific cleavage of this kind (i.e after any
basic residue) with enterokinase ???
thanks for help
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