denaturing polyacrilamid gel for DNA

Tal Yaron shkootz at altavista.net
Thu Jul 6 05:07:32 EST 2000


Hi everybody

I tried to denature complementary oligos (about 17 bps complementation)
with the procedure of heating to 95c for 5 minutes and instantly
entering them to ice basket. then loading them on a 45c heated
polyacrilamid gel [6M urea]. but most of the loading got carried away
from the gel, right after I entered it.

do you know about other procedures for DNA denaturing and
electrophoresis on polyacrilamid?

Thank you
Tal










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