S-35 and autoradiography

Mark Farrar MICMDF at leeds.ac.uk
Thu Jul 6 04:50:13 EST 2000


>
>Are you sure this is more sensitive than conventional
>fluorograpy?
>It's been awhile, but back in my PPO/DMSO days ISTR getting about
>that exposure time on film with about that many cpm of 35S.
>
>Nick
>
>
>Nick Theodorakis
>
>nicholas_theodorakis at urmc.rochester.edu
>

I've used phosphorimaging with much less cpm and exposure times depending on 
what proteins are being analysed, I was just giving an example of one 
experiment.






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