PCR directly from eukaryotic cell without DNA extraction

Chris LaRosa clarosa at biocomp.unl.edu
Thu Jul 6 09:08:12 EST 2000



biobiobio_2000 wrote:
> 
> Hi all,
> 
> It is known that PCR could be done directly on bacteria cells. However, is
> it any equivalent protocol for that performed on eukaryotic cells? Please
> direct me to any resource about that. Thank you.
> 
On can do colony pcr with yeast.  Its harder than probing for a plasmid,
but pcr off t he genomic dna works. Animal cells? dont know.






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