Plasmid miniprep from Agrobacterium

Tomek alaska9 at friko5.onet.pl
Fri Jul 7 03:58:24 EST 2000


Hi,

I`m lookin for efficient method of miniprep binary vector from
Agrobacterium tumefaciens. After transformation I have very many
colonies, but I can`t prepare plasmid pbi121 from colonies. Because my
negative control dish is completly clear, I think, that my plasmid is
in Agrobact. and I can`t isolate it.

Thanks in advance

Tomek







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