DNA contanmination in RNA prep

Chris LaRosa clarosa at biocomp.unl.edu
Fri Jul 7 12:12:21 EST 2000


> 
> I've used the DNase I, RNase free from Roche but some how the DNA
> just seems to be very recalcitrant.
>
hmm... perhaps an additional LiCl2 ppt step where DNA/RNA can be
separated, then DNAse.  Why is the DNA recalcitrant? You may need to
protease treat the DNA which might be protected.






More information about the Methods mailing list