DNA contanmination in RNA prep

Chris LaRosa clarosa at biocomp.unl.edu
Fri Jul 7 12:12:21 EST 2000

> I've used the DNase I, RNase free from Roche but some how the DNA
> just seems to be very recalcitrant.
hmm... perhaps an additional LiCl2 ppt step where DNA/RNA can be
separated, then DNAse.  Why is the DNA recalcitrant? You may need to
protease treat the DNA which might be protected.

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