PCR ?

David Keszenman-Pereyra D.K-Pereyra at sheffield.ac.uk
Sat Jul 8 04:10:43 EST 2000

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I want to integrate a selectable cassette randomly into the genome. 
Then I want to sequence the flanking regions of the integration site.
Is there any PCR method to do it quickly ????? (single primer 
amplification ?????)


David Keszenman-Pereyra
University of Sheffield
Department of Molecular Biology & BIotechnology
Firth Court, Western Bank, Sheffield S10 2TN
UK 




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