PCR ?

Chris LaRosa clarosa at biocomp.unl.edu
Sat Jul 8 10:44:55 EST 2000



David Keszenman-Pereyra wrote:
> 
> I want to integrate a selectable cassette randomly into the genome.
> Then I want to sequence the flanking regions of the integration site.
> Is there any PCR method to do it quickly ????? (single primer
> amplification ?????)
> 
> 
Plasmid rescue and inverse pcr strategy ... just a speculation.   also
look at vectorette technology.






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