Gene construction advice needed

Tal Yaron shkootz at altavista.net
Sun Jul 9 07:20:35 EST 2000


I have hard that GIBCO BRL can build oligos of up to 15,000 bs.
you can try there.

http://www.lifetech.com/content.cfm?pageid=277&cfid=111889&cftoken=25921393


"Joseph C. Bagshaw" wrote:

> I'm looking for advice on a gene construction project.  I'm trying to
> assemble a 219 bp gene from oligonucleotides.  I designed the oligos so
> that each overlaps the next by ten complementary bases.  The plan then is
> to fill the gaps, then ligate.  I can get perfect results with any two
> adjacent oligos, but when I try to assemble the whole, instead of a 219 bp
> band I get a smear.  Any advice or direction to any source of information
> would be greatly appreciated.  I have not found any reference or "how
> to" source, like "Idiot's Guide to Gene Construction".  Please mail to the
> address below or post here.
>
> ********************  HAVE GENES, WILL TRAVEL  ********************
> Joe Bagshaw, Worcester Polytechnic Institute
> jbagshaw at wpi.edu
> Roadkill on the information superhighway.

--
Tal Yaron
At Prof. Gideon Fleminger's lab

Department of Biotechnology
Wise Faculty of Life Science
Tel Aviv University
Israel







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