Plasmid miniprep from Agrobacterium

Tomek alaska9 at friko5.onet.pl
Mon Jul 10 07:36:16 EST 2000


On Fri, 07 Jul 2000 22:51:50 GMT, ckafer at iastate.edu (Chris Kafer)
wrote:


Thanks. I think that will be the best way.

Tomek

 The way we screen is to
>do a miniprep of the Agro (alkaline lysis phenol method) and then
>transform  E. coli with a few microliters of this and plate.  Now you
>should be able to do a regular miniprep of a culture grown from a
>single E. coli colony.  If the E. coli carries the plasmid with insert
>then your Agro did too!
>
>Yes, takes an extra day or 2 but saves aggravation.
>
>Good Luck!







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