Plasmid miniprep from Agrobacterium
alaska9 at friko5.onet.pl
Mon Jul 10 07:36:16 EST 2000
On Fri, 07 Jul 2000 22:51:50 GMT, ckafer at iastate.edu (Chris Kafer)
Thanks. I think that will be the best way.
The way we screen is to
>do a miniprep of the Agro (alkaline lysis phenol method) and then
>transform E. coli with a few microliters of this and plate. Now you
>should be able to do a regular miniprep of a culture grown from a
>single E. coli colony. If the E. coli carries the plasmid with insert
>then your Agro did too!
>Yes, takes an extra day or 2 but saves aggravation.
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